pC015 - dLwCas13a-NF
(Plasmid #91905)

• Purpose: Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
• Depositing Lab: Feng Zhang
• Publication: Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature24049.

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 5876
• Total vector size (bp): 10622
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dLwCas13a
• Alt name: dead LwaCas13a
• Alt name: dead LwC2c2
• Alt name: dCas13a
• Species: H. sapiens (human); Leptotrichia wadei
• Insert Size (bp): 4806
• Mutation: R474A and R1046A
• Promoter: CAG
• Tags / Fusion Proteins:
  • eGFP, ZF CCR5TC, KRAB-A, NLS (C terminal on insert)
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F

Resource Information

• Supplemental Documents:
  • pr0218pcag-dc2c2-egfp-ccr5tc-nls.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry