pC016 - LwCas13a guide expression backbone with U6 promoter Citations (10)

Originally described in: RNA targeting with CRISPR-Cas13.
Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F Nature. 2017 Oct 4. doi: 10.1038/nature24049. PubMed Journal

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Articles Citing pC016 - LwCas13a guide expression backbone with U6 promoter

Articles
Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033. PubMed Associated Plasmids pXR001: EF1a-CasRx-2A-EGFP pXR002: EF1a-dCasRx-2A-EGFP pXR003: CasRx gRNA cloning backbone pXR004: CasRx pre-gRNA cloning backbone
Programmable Inhibition and Detection of RNA Viruses Using Cas13. Freije CA, Myhrvold C, Boehm CK, Lin AE, Welch NL, Carter A, Metsky HC, Luo CY, Abudayyeh OO, Gootenberg JS, Yozwiak NL, Zhang F, Sabeti PC. Mol Cell. 2019 Dec 5;76(5):826-837.e11. doi: 10.1016/j.molcel.2019.09.013. Epub 2019 Oct 10. PubMed
A CRISPR/Cas13-based approach demonstrates biological relevance of vlinc class of long non-coding RNAs in anticancer drug response. Xu D, Cai Y, Tang L, Han X, Gao F, Cao H, Qi F, Kapranov P. Sci Rep. 2020 Feb 4;10(1):1794. doi: 10.1038/s41598-020-58104-5. PubMed
CRISPR-based RNA-binding protein mapping in live cells. Chen B, Shi H, Zhang J, Zhou C, Han M, Jiang W, Lai Y, Tu X, Li H. Biochem Biophys Res Commun. 2021 Oct 29;583:79-85. doi: 10.1016/j.bbrc.2021.10.059. PubMed
Parallel functional assessment of m(6)A sites in human endodermal differentiation with base editor screens. Cheng W, Liu F, Ren Z, Chen W, Chen Y, Liu T, Ma Y, Cao N, Wang J. Nat Commun. 2022 Jan 25;13(1):478. doi: 10.1038/s41467-022-28106-0. PubMed
Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system. Chen M, Sui T, Yang L, Qian Y, Liu Z, Liu Y, Wang G, Lai L, Li Z. Biosens Bioelectron. 2022 May 15;204:114074. doi: 10.1016/j.bios.2022.114074. Epub 2022 Feb 4. PubMed
Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Tian S, Liu Y, Appleton E, Wang H, Church GM, Dong M. Cell Rep. 2022 Mar 8;38(10):110476. doi: 10.1016/j.celrep.2022.110476. PubMed
Programmable protein expression using a genetically encoded m(6)A sensor. Marayati BF, Thompson MG, Holley CL, Horner SM, Meyer KD. Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3. PubMed Associated Plasmids pGEMS-II pGEMS-II-PEST pGEMS-II-dCas13-APO1-YTH pU6-PspCas13b-GEMSgRNA2
dCas13-mediated translational repression for accurate gene silencing in mammalian cells. Apostolopoulos A, Kawamoto N, Chow SYA, Tsuiji H, Ikeuchi Y, Shichino Y, Iwasaki S. Nat Commun. 2024 Mar 11;15(1):2205. doi: 10.1038/s41467-024-46412-7. PubMed Associated Plasmids pCAGEN-PspCas13b-NES-mCherry pCAGEN-dPspCas13b-NES-mCherry pCAGEN-dPspCas13b-4EHP-NES-mCherry pC016-gPsp-EGFP_start_codon
Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 system. Manjunath LE, Singh A, Devi Kumar S, Vasu K, Kar D, Sellamuthu K, Eswarappa SM. EMBO Rep. 2024 Apr;25(4):2118-2143. doi: 10.1038/s44319-024-00115-8. Epub 2024 Mar 18. PubMed

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