PurposeExpresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||92031||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5288
- Total vector size (bp) 7742
Modifications to backboneIRES element between mCherry and XhoI
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
/ Fusion Protein
- GalBD-VP16AD (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XmaI (not destroyed)
- 5′ sequencing primer custom
- 3′ sequencing primer custom (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CRY-GalVP16 (B695) was a gift from Chandra Tucker (Addgene plasmid # 92031 ; http://n2t.net/addgene:92031 ; RRID:Addgene_92031)
For your References section:Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL. Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260. 10.1093/nar/gkx260 PubMed 28431041