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CRY-GalVP16 (B695)
(Plasmid #92031)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92031 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherryC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5288
  • Total vector size (bp) 7742
  • Modifications to backbone
    IRES element between mCherry and XhoI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Tag / Fusion Protein
    • GalBD-VP16AD (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer custom
  • 3′ sequencing primer custom
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY-GalVP16 (B695) was a gift from Chandra Tucker (Addgene plasmid # 92031 ; http://n2t.net/addgene:92031 ; RRID:Addgene_92031)
  • For your References section:

    Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL. Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260. 10.1093/nar/gkx260 PubMed 28431041