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Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2.
Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL
Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
92030CRY-GalVP16l (B694)expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)
92031CRY-GalVP16 (B695)Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.
92032CRY-tTA (B702)Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator
92035CRY-Gal∆DD (B1013)Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES
92036CIB-VP16 (B1014)Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.
92037CIB-VP64 (B1016)Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)