PurposeExpresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||92036||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5462
- Total vector size (bp) 6779
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)1044
Entrez GeneCIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
- Promoter T7
/ Fusion Protein
- Fusion of plant CIB1 (intact NLS) with VP16 activation domain
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer custom
- 3′ sequencing primer custom (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CIB-VP16 (B1014) was a gift from Chandra Tucker (Addgene plasmid # 92036 ; http://n2t.net/addgene:92036 ; RRID:Addgene_92036)
For your References section:Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL. Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260. 10.1093/nar/gkx260 PubMed 28431041