This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p-mCherry-C1-iCoChR++
(Plasmid #98173)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98173 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4714
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synthetic construct iCoChR++ gene
  • Alt name
    iCoChR++
  • Alt name
    CoChR
  • Species
    Synthetic
  • Insert Size (bp)
    915
  • Mutation
    A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T246N, N258Q, Q273S
  • Promoter CMV (+enhancer)
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer EGFP-N1-F: GAGGTCTATATAAGCAGAGC or CMV-F: GCAAATGGGCGGTAGGCGT
  • 3′ sequencing primer EGFP-C1-R: AACCATTATAAGCTGCAATAAAC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-mCherry-C1-iCoChR++ was a gift from Peter Hegemann (Addgene plasmid # 98173)
  • For your References section:

    Artificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity. Wietek, Jonas, Silvia Rodriguez-Rozada, Janine Tutas, Federico Tenedini, Christiane Grimm, Thomas Oertner, Peter Soba, Peter Hegemann, J. Simon Wiegert. biorxiv /10.1101/156422