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pLgw EcoDam-V5-SRF
(Plasmid #98602)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98602 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLgw EcoDam-V5-RFC1
  • Backbone manufacturer
    PMID 9733862
  • Vector type
    Mammalian Expression, Lentiviral ; DamID

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRF
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Srf (a.k.a. AW049942, AW240594)
  • Promoter Heat Shock Minimal Promoter
  • Tag / Fusion Protein
    • Dam (DNA adenine methyltransferase) and V5 (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLgw EcoDam-V5-SRF was a gift from Richard Harvey (Addgene plasmid # 98602 ; http://n2t.net/addgene:98602 ; RRID:Addgene_98602)
  • For your References section:

    NKX2-5 mutations causative for congenital heart disease retain functionality and are directed to hundreds of targets. Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA, Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, Harvey RP. Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942. 10.7554/eLife.06942 PubMed 26146939