pAimR
(Plasmid #98687)

• Purpose: Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag
• Depositing Lab: Rotem Sorek
• Publication: Erez et al Nature. 2017 Jan 26;541(7638):488-493. doi: 10.1038/nature21049. Epub 2017 Jan 18.

Backbone

• Vector backbone: pBs1C
• Backbone size w/o insert (bp): 6061
• Total vector size (bp): 7386
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AimR
• Species: Phage phi3T
• Insert Size (bp): 1325
• GenBank ID: KY030782
• Promoter: Native
• Tag / Fusion Protein:
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: pstI (unknown if destroyed)
• 5′ sequencing primer: aaGAATTCCTCATTGTGTTTAGGTAAAATAAGAAATTC
• 3′ sequencing primer: aaCTGCAGTTAGTGGTGGTGGTGGTGGTGAATAGAGATAAGGTTTAATAATTCAAG

Resource Information

• Supplemental Documents:
  • pAimR_addgene.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ampicillin and chloramphenicol selection should be used if plasmid is used for Bacillus transformation.