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STARR-seq luciferase validation vector_ORI_AGAP1
(Plasmid #99300)


Item Catalog # Description Quantity Price (USD)
Plasmid 99300 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    STARR-seq luciferase validation vector_ORI
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3977
  • Vector type
    Mammalian Expression, Luciferase ; STARR-seq Luciferase validation vector

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    enhancer; chr2: 236735694-236737193
  • Species
    H. sapiens (human)
  • Insert Size (bp)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Depositor Comments

This vector has the AGAP1 enhancer cloned downstream of the reporter gene; The origin of replication (ORI) substitutes the core promoter . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STARR-seq luciferase validation vector_ORI_AGAP1 was a gift from Alexander Stark (Addgene plasmid # 99300 ; ; RRID:Addgene_99300)
  • For your References section:

    Resolving systematic errors in widely used enhancer activity assays in human cells. Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A. Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. 10.1038/nmeth.4534 PubMed 29256496