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Resolving systematic errors in widely used enhancer activity assays in human cells.
Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A
Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
99291hSTARR-seq_mCP vectorVector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99292hSTARR-seq_SCP1 vectorVector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99293hSTARR-seq_TTF2 vectorVector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99294hSTARR-seq_SULT1C vectorVector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99295hSTARR-seq_OCT4 3UTR vectorVector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99296hSTARR-seq_ORI vectorVector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99297STARR-seq luciferase validation vector_ORI_emptyLuciferase validation vector; empty vector without insert to determine baseline
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99298STARR-seq luciferase validation vector_mP_emptyLuciferase validation vector; empty vector without insert to determine baseline
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99299STARR-seq luciferase validation vector_SCP1_emptyLuciferase validation vector; empty vector without insert to determine baseline
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99300STARR-seq luciferase validation vector_ORI_AGAP1Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99301STARR-seq luciferase validation vector_mP_AGAP1Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99302STARR-seq luciferase validation vector_SCP1_AGAP1Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99303STARR-seq luciferase validation vector_ORI_GTSE1Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99304STARR-seq luciferase validation vector_mP_GTSE1Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99305STARR-seq luciferase validation vector_SCP1_GTSE1Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99306STARR-seq luciferase validation vector_ORI_IGF1RLuciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99307STARR-seq luciferase validation vector_mP_IGF1RLuciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99308STARR-seq luciferase validation vector_SCP1_IGF1RLuciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99309STARR-seq luciferase validation vector_ORI_SV40Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99310STARR-seq luciferase validation vector_mP_SV40Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99311STARR-seq luciferase validation vector_SCP1_SV40Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99312STARR-seq luciferase validation vector_ORI_CMVLuciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99313STARR-seq luciferase validation vector_mP_CMVLuciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99314STARR-seq luciferase validation vector_SCP1_CMVLuciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99315STARR-seq luciferase validation vector_ORI_neg.contNegative control for luciferase validation vectors
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99316hSTARR-seq_SCP1 vector_blocking 1Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99317hSTARR-seq_SCP1 vector_blocking 2Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99318hSTARR-seq_SCP1 vector_blocking 3Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99319hSTARR-seq_SCP1 vector_blocking 4Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.
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99320STARR-seq luciferase INF enhancer vector_ORI_IFI27Luciferase validation vector with IFI27 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99321STARR-seq luciferase INF enhancer vector_ORI_IFIT2Luciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99322STARR-seq luciferase INF enhancer vector_ORI_ISG15Luciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99323STARR-seq luciferase INF enhancer vector_ORI_MX1Luciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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99324STARR-seq luciferase INF enhancer vector_ORI_OAS3Luciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
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