pMarVF1
(Plasmid #99579)

• Purpose: Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114
• Depositing Lab: Mark Mandel
• Publication: Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17284-9. doi: 10.1073/pnas.1415957111. Epub 2014 Nov 17.

Backbone

• Vector backbone: pSAM
• Backbone manufacturer: Jeffrey I. Gordon, PMID: 19748469
• Backbone size w/o insert (bp): 3608
• Total vector size (bp): 4552
• Modifications to backbone: nrdR promoter was added using BamHI/NdeI sites to drive expression of himar1C9 transposase
• Vector type: Suicide vector in V. fischeri ES114
• Selectable markers: Erythromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): S17-1 λpir
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
• Insert Size (bp): 944

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pMarVF1_Map KW.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry