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pGL4.20-HIF1A prom
(Plasmid #40173)


Item Catalog # Description Quantity Price (USD)
Plasmid 40173 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Modifications to backbone
    addition of HIF1A (human) promoter fragment
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    HIF1A promoter fragment
  • Species
    H. sapiens (human)
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Hind3 (not destroyed)
  • 5′ sequencing primer pGL4 sequencing oligo
  • 3′ sequencing primer internal oligo
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.20-HIF1A prom was a gift from Alex Minella (Addgene plasmid # 40173 ; ; RRID:Addgene_40173)
  • For your References section:

    Hypoxia-inducible factor 1 is activated by dysregulated cyclin E during mammary epithelial morphogenesis. Sengupta T, Abraham G, Xu Y, Clurman BE, Minella AC. Mol Cell Biol. 2011 Sep;31(18):3885-95. Epub 2011 Jul 11. 10.1128/MCB.05089-11 PubMed 21746877