Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40172||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backboneaddition of HIF1A promoter fragment
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert nameHIF1A promoter fragment
SpeciesH. sapiens (human)
Insert Size (bp)2208
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Hind3 (not destroyed)
- 5′ sequencing primer pGL2 sequencing
- 3′ sequencing primer pGL2 sequencing (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2B-HIF1A prom was a gift from Alex Minella (Addgene plasmid # 40172 ; http://n2t.net/addgene:40172 ; RRID:Addgene_40172)
For your References section:Hypoxia-inducible factor 1 is activated by dysregulated cyclin E during mammary epithelial morphogenesis. Sengupta T, Abraham G, Xu Y, Clurman BE, Minella AC. Mol Cell Biol. 2011 Sep;31(18):3885-95. Epub 2011 Jul 11. 10.1128/MCB.05089-11 PubMed 21746877