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pGL2B-HIF1A prom
(Plasmid #40172)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40172 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2B
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    addition of HIF1A promoter fragment
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIF1A promoter fragment
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2208

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Hind3 (not destroyed)
  • 5′ sequencing primer pGL2 sequencing
  • 3′ sequencing primer pGL2 sequencing
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2B-HIF1A prom was a gift from Alex Minella (Addgene plasmid # 40172 ; http://n2t.net/addgene:40172 ; RRID:Addgene_40172)
  • For your References section:

    Hypoxia-inducible factor 1 is activated by dysregulated cyclin E during mammary epithelial morphogenesis. Sengupta T, Abraham G, Xu Y, Clurman BE, Minella AC. Mol Cell Biol. 2011 Sep;31(18):3885-95. Epub 2011 Jul 11. 10.1128/MCB.05089-11 PubMed 21746877