Axel Brunger Lab Plasmids


The Axel Brunger Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. Arac D, Boucard AA, Bolliger MF, Nguyen J, Soltis SM, Sudhof TC, Brunger AT EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26.
A potent peptidomimetic inhibitor of botulinum neurotoxin serotype A has a very different conformation than SNAP-25 substrate. Zuniga JE, Schmidt JJ, Fenn T, Burnett JC, Arac D, Gussio R, Stafford RG, Badie SS, Bavari S, Brunger AT Structure. 2008 Oct 8. 16(10):1588-97.
Structures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions. Arac D, Boucard AA, Ozkan E, Strop P, Newell E, Sudhof TC, Brunger AT Neuron. 2007 Dec 20. 56(6):992-1003.
Central pore residues mediate the p97/VCP activity required for ERAD. DeLaBarre B, Christianson JC, Kopito RR, Brunger AT Mol Cell. 2006 May 19. 22(4):451-62.
Structural basis of FFAT motif-mediated ER targeting. Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT Structure. 2005 Jul . 13(7):1035-45.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12281 pBD-0005 VCP K315L (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12339 pBD-0071 VCP K251A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12344 pBD-0010 VCP E305Q (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12345 pBD-0016 VCP R313K (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp RP23-124L1.5, 3110001E05, CDC48, p97, p97/VCP
12346 pBD-0013 VCP R313A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12347 pBD-0006 VCP T316V (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12348 pBD-0009 VCP T317Q (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12349 pBD-0070 VCP H317N (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12350 pBD-0069 VCP H317A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12351 pBD-0008 VCP E319L (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12352 pBD-0007 VCP E319Q (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12353 pBD-0018 VCP R322A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12354 pBD-0020 VCP R322K (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12355 pBD-0022 VCP R323A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12356 pBD-0024 VCP R323K (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12357 pBD-0032 VCP R362A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12358 pBD-0035 VCP W551A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12359 pBD-0039 VCP F552A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12360 pBD-0037 VCP W551A F552A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12361 pBD-0072 VCP E578Q (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12362 pBD-0050 VCP R586A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12363 pBD-0003 VCP R586K (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12364 pBD-0026 VCP D592N (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12365 pBD-0028 VCP D598N (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12366 pBD-0045 VCP R599A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12367 pBD-0011 VCP R599K (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12368 pBD-0030 VCP R638A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12369 pBD-0082 VCP K524A (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp
12373 pBD-0001 VCP (Mus musculus) Bacterial Expression DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp RP23-124L1.5, 3110001E05, CDC48, p97, p97/VCP
13395 pGEX-T-rnVAPA-1-219 VAP-A (Rattus norvegicus) Bacterial Expression Kaiser et al Structure. 2005 Jul . 13(7):1035-45. pGEX-4T Vapa
13396 pGEX-T-rnVAPA-1-125 VAP-A (Rattus norvegicus) Bacterial Expression Kaiser et al Structure. 2005 Jul . 13(7):1035-45. pGEX-4T Vapa
18874 pEGFP-N1-VAPA(1-242) VAP-A (Rattus norvegicus) Mammalian Expression Kaiser et al Structure. 2005 Jul . 13(7):1035-45. pEGFP-N1 Vapa
18875 pEGFP-N1-VAPA(1-242) K87D/M89D VAP-A (Rattus norvegicus) Mammalian Expression Kaiser et al Structure. 2005 Jul . 13(7):1035-45. pEGFP-N1 Vapa
20172 rNL1(46-638) Neuroligin 1 (Rattus norvegicus) Insect Expression Arac et al Neuron. 2007 Dec 20. 56(6):992-1003. pACGP67a Nlgn1
20173 rNrx1beta(47-299) Neurexin 1 beta (Rattus norvegicus) Insect Expression Arac et al Neuron. 2007 Dec 20. 56(6):992-1003. pACGP67a Nrxn1 Nrxn1b
31602 BoNT/A-LC BoNT/A LC Bacterial Expression Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97. pBN3
37847 pAcGP67A-rCL1-HormR-GAIN CL1 (Rattus norvegicus) Baculovirus Transfer Vectors Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26. pAcGP67A Lphn1 CL1BA
37848 pAcGP67A-hBAI3-HormR-GAIN BAI3 (Homo sapiens) Baculovirus Transfer Vectors Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26. pAcGP67A BAI3 KIAA0550, MGC133100