Taek_Soon Lee Lab Plasmids


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Title Authors Publication
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ, Lee TS Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Satoh Y, Tajima K, Munekata M, Keasling JD, Lee TS Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
35150 pBbA5c-MevT-MBIS AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA (Saccharomyces cerevisiae) Bacterial Expression, Synthetic Biology Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c
35151 pBbA5c-MevT(CO)-MBIS(CO, ispA) AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae) Bacterial Expression, Synthetic Biology Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c
35152 pBbA5c-MevT(CO)-T1-MBIS(CO, ispA) AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae) Bacterial Expression, Synthetic Biology Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c
35153 pTrcAgBIS (CO) Bisabolene Synthase Bacterial Expression, Synthetic Biology Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pTrc99a
35524 pRSLeu2d-BISopt Bisabolene Synthase Yeast Expression, Synthetic Biology Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pRS425ADS-Leu2d
47048 pJBEI-6409 Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae) Bacterial Expression, Synthetic Biology ; BglBrick Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli pBbA5c
47049 pJBEI-6410 Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae) Bacterial Expression, Synthetic Biology ; BglBrick Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli pBbA5a
47050 pJBEI-6411 ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase (Other) Bacterial Expression, Synthetic Biology ; BglBrick Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli pBbB8k
59945 pBbE1k-2 dihydropterine reductase, pterin-4a-carbinolamine dehydratase Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human pBbE1k