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Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65591AP56-5co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
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65593AP56-6co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
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65594AP56-7co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
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65596AP56-8co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
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65597AP54-3co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
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65599AP54-5co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
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66085APq5271co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF
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66086APq5210co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF
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66087AP303-3co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
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66088AP303-4co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
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66089AP332-2co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
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66090AP332-3co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
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66091AP332-4co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
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66092AP324-2co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
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66093AP324-3co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
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66094AP324-4co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
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66095AP334-2co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
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66096AP334-5co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
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66097AP334-6co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
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66099APCSD 53co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2
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66100APCSD 54co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2
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