Purposeco-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66086||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGoldstein lab Plasmid 47549
Vector typeWorm Expression, CRISPR
Growth in Bacteria
Gene/Insert namesgRNA for APa4-2
- Promoter U6
- Cloning method Unknown
- 5′ sequencing primer tatgaaatgcctacaccctctc (Common Sequencing Primers)
From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:APq5210 was a gift from Geraldine Seydoux (Addgene plasmid # 66086 ; http://n2t.net/addgene:66086 ; RRID:Addgene_66086)
For your References section:Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23. 10.1534/genetics.114.170423 PubMed 25249454