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Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters.
MacKay JL, Kumar S
Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84605pSLIK CA Rac1Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus
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84642pSLIK DN Rac1Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus
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84643pCuo CA Rac1constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.
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84644pCuo DN Rac1dominant negative Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.
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84646pSLIK DN RhoALentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus
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112894pSLIK CA RhoApromoter-inducible expression of constitutively-active RhoA
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