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Charles Gersbach Lab: TMG reporters
Gersbach
Unpublished

Plasmids from Article

ID Plasmid Purpose  
69545tetO-GFPnls-mPGK-rtTA-IRES-PuroTet-On system expressing GFP tagged with 2x nuclear localization signals.
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69546tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-PuroTet-On system expressing MyoD-2A-GFP-2xNLS.
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69547Myog'-2A-mCherryNLS-PGK-PurodtkDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
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69548Myog'-2A-mCherryNLS-PGK-PuroDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
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69549pZDonor-U6-myogenin stop codon gRNAExpresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
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69550FU-H2B-GFP-IRES-PuroLentiviral, selectable fluorescent GFP tagging of nuclei.
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