Connexin Type and Fluorescent Protein-fusion Tag Determine Structural Stability of Gap Junction Plaques.
Stout RF Jr, Snapp EL, Spray DC
J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979. PubMed Article
Stout RF Jr, Snapp EL, Spray DC
J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 69007 | Cx43-msfGFP | Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter. |
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| 69016 | Cx26-msfGFP | Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter. |
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| 69019 | Cx30-msfGFP | Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter. |
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| 69020 | EBFP2-Cx43 | Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter. |
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| 69021 | EBFP2-Cx26 | Expresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter. |
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| 69022 | EBFP2-Cx30 | Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter. |
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| 69023 | Cx43K258stop-msfGFP | Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter. |
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| 69024 | msfGFP-Cx43 | Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter. |
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| 69025 | sfGFP-Cx43K258stop | Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter. |
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| 69026 | Cx26-sfGFP | Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter. |
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| 70053 | Cx43-sfGFP | Expresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter. |
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