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A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic Reticulum Calcium Store.
Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X, Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
PLoS One. 2015 Oct 9;10(10):e0139273. doi: 10.1371/journal.pone.0139273. eCollection 2015. PubMed

Plasmids from Article

ID Plasmid Purpose
63884pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDELAn AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.
63885pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDELAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.
63886pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDELAn AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.
63887pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDELAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.
65227pMOS003-lenti-CMV-GCaMPerER calcium sensor for lentivirus production

Antibodies from Article