pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL
PurposeAn AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63886||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneAddgene Plasmid #50465
Backbone manufacturerBryan Roth
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameER-localized GCaMP3(wt)
- Promoter human SYN1
- Cloning method Restriction Enzyme
- 5′ cloning site bamhi (unknown if destroyed)
- 3′ cloning site ecori (unknown if destroyed)
- 5′ sequencing primer hSYN1 F417 actcagcgctgcctcagtct
- 3′ sequencing primer WPRE_R1 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL was a gift from Brandon Harvey (Addgene plasmid # 63886 ; http://n2t.net/addgene:63886 ; RRID:Addgene_63886)
For your References section:A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic Reticulum Calcium Store. Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X, Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK. PLoS One. 2015 Oct 9;10(10):e0139273. doi: 10.1371/journal.pone.0139273. eCollection 2015. PONE-D-15-20579 [pii] PubMed 26451944