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Identification and characterization of essential genes in the human genome.
Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES, Sabatini DM
Science. 2015 Oct 15. pii: aac7041.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
70265pMXs-C3orf17-gfpExpresses C3orf17 in mammalian cells with GFP reporter.
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70266pMXs-C9orf114-gfpExpresses C9orf114 in mammalian cells with GFP reporter.
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70645pMXs-C16orf80-flagExpresses C16orf80 in mammalian cells with FLAG-tag epitope.
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70646pMXs-C3orf17-flagExpresses C3orf17 in mammalian cells with FLAG-tag epitope.
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70647pMXs-C9orf114-flagExpresses C9orf114 with FLAG-tag epitope.
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70648pMXs-DDX3XExpresses WT DDX3X in mammalian cells under blasticidin selection.
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70649pTagRFP-C1-FibrillarinExpresses Fibrillarin nucleolar marker with TurboRFP reporter.
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70650lentiCRISPR - C16orf80 sgRNA 2Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70651lentiCRISPR - C16orf80 sgRNA 1Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70652lentiCRISPR - C3orf17 sgRNA 1Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70653lentiCRISPR - C3orf17 sgRNA 2Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70654lentiCRISPR - C9orf114 sgRNA 2Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70655lentiCRISPR - C9orf114 sgRNA 1 Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70656lentiCRISPR - DDX3Y sgRNA 1Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70657lentiCRISPR - DDX3Y sgRNA 2Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
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70658lentiCRISPR - Amplicon, BCR/ABL sgRNA 2Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.
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70659lentiCRISPR - Amplicon, BCR/ABL sgRNA 1Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
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70660lentiCRISPR - Amplicon, JAK2 sgRNA 2Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
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70661lentiCRISPR - AAVS1 sgRNAExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone
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70662lentiCRISPR - CTRL sgRNAExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone
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70679lentiCRISPR - Amplicon, JAK2 sgRNA 1 Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
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