Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms.
Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb RE, Webb RI, Teasdale RD, Parton RG
Dev Cell. 2015 Nov 23;35(4):513-25. doi: 10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
67650mKate2-P2A-APEX2-GBPMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
Loading...
67651APEX2-GBPMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).
Loading...
67652nls-EGFPMammalian expression vector driving nls-EGFP.
Loading...
67653BTK-APEX2-P2A-mKate2Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
Loading...
67654AKT-APEX2-P2A-mKate2Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
Loading...
67662mKate2-P2A-APEX2-TAPP1-PHMammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
Loading...
67663mKate2-P2A-APEX2-2xFYVE_hrsMammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
Loading...
67664mKate2-P2A-APEX2-OSBPMammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
Loading...
67665mKate2-P2A-APEX2-ML1N*2Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
Loading...
67666mKate2-P2A-APEX2-2xML1N(7Q)Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
Loading...
67667Cav1-miniSOGMammalian expression vector driving dog Caveolin1-minSOG
Loading...
67668Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherryZebrafish expression vector for ubiquitous expression of APEX2-GBP
Loading...
67669pME-APEX2-NSGateway entry clone containing APEX2 with no stop codon
Loading...
67670pME-mKate-P2A-APEX2-NSGateway entry clone containing mKate2-P2A-APEX2 with no stop codon
Loading...
67671p3E-APEX2-P2A-mKate2Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2
Loading...
67672p3E-GBPMultisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)
Loading...
67673pME-BTK-PHGateway entry clone containing BTK-PH with no stop codon
Loading...
67674pME-AKT-PHGateway entry clone containing AKT-PH with no stop codon
Loading...
67675p3E-TAPP1-PHMultisite gateway entry clone for adding C-terminal fusions of TAPP1-PH
Loading...
67676p3E-2xFYVE_hrsMultisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs
Loading...
67677p3E-OSBPMultisite gateway entry clone for adding C-terminal fusions of OSBP
Loading...
67678p3E-ML1N*2Multisite gateway entry clone for adding C-terminal fusions of ML1N*2
Loading...
67679p3E-ML1N*2(7Q)Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)
Loading...
67796ML1Nmut_in_pEGFP-C1Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP
Loading...
67797ML1Nwt_in_pEGFP-C1Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP
Loading...
71282HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherryZebrafish expression vector for heat-shock inducible expression of APEX2-GBP
Loading...
71283pME-2PH-PLCdMultisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta
Loading...
712842PH-PLCd-APEX2-P2A-mKate2Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter
Loading...