Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms.
Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb RE, Webb RI, Teasdale RD, Parton RG
Dev Cell. 2015 Nov 23;35(4):513-25. doi: 10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12. PubMed Article
Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb RE, Webb RI, Teasdale RD, Parton RG
Dev Cell. 2015 Nov 23;35(4):513-25. doi: 10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 67650 | mKate2-P2A-APEX2-GBP | Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter. |
| 67651 | APEX2-GBP | Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). |
| 67652 | nls-EGFP | Mammalian expression vector driving nls-EGFP. |
| 67653 | BTK-APEX2-P2A-mKate2 | Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter. |
| 67654 | AKT-APEX2-P2A-mKate2 | Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter. |
| 67662 | mKate2-P2A-APEX2-TAPP1-PH | Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter. |
| 67663 | mKate2-P2A-APEX2-2xFYVE_hrs | Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter. |
| 67664 | mKate2-P2A-APEX2-OSBP | Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter. |
| 67665 | mKate2-P2A-APEX2-ML1N*2 | Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter. |
| 67666 | mKate2-P2A-APEX2-2xML1N(7Q) | Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter. |
| 67667 | Cav1-miniSOG | Mammalian expression vector driving dog Caveolin1-minSOG |
| 67668 | Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry | Zebrafish expression vector for ubiquitous expression of APEX2-GBP |
| 67669 | pME-APEX2-NS | Gateway entry clone containing APEX2 with no stop codon |
| 67670 | pME-mKate-P2A-APEX2-NS | Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon |
| 67671 | p3E-APEX2-P2A-mKate2 | Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2 |
| 67672 | p3E-GBP | Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP) |
| 67673 | pME-BTK-PH | Gateway entry clone containing BTK-PH with no stop codon |
| 67674 | pME-AKT-PH | Gateway entry clone containing AKT-PH with no stop codon |
| 67675 | p3E-TAPP1-PH | Multisite gateway entry clone for adding C-terminal fusions of TAPP1-PH |
| 67676 | p3E-2xFYVE_hrs | Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs |
| 67677 | p3E-OSBP | Multisite gateway entry clone for adding C-terminal fusions of OSBP |
| 67678 | p3E-ML1N*2 | Multisite gateway entry clone for adding C-terminal fusions of ML1N*2 |
| 67679 | p3E-ML1N*2(7Q) | Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q) |
| 67796 | ML1Nmut_in_pEGFP-C1 | Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP |
| 67797 | ML1Nwt_in_pEGFP-C1 | Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP |
| 71282 | HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry | Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP |
| 71283 | pME-2PH-PLCd | Multisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta |
| 71284 | 2PH-PLCd-APEX2-P2A-mKate2 | Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter |