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DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.
Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
PubMed

Plasmids from Article

ID Plasmid Purpose  
72619pDECKO_GFPExpresses two gRNAs targeting GFP
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72620pDECKO_Malat1_AExpresses two gRNAs targeting MALAT1 promoter
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72621pDECKO_Malat1_BExpresses two gRNAs targeting MALAT1 promoter
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72622pDECKO_Malat1_CExpresses two gRNAs targeting MALAT1 promoter
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72623pDECKO_Malat1_DExpresses two gRNAs targeting MALAT1 promoter
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72624pDECKO_Malat1_EExpresses two gRNAs targeting MALAT1 promoter
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72626pDECKO_TFRC_BExpresses two gRNAs targeting the TFRC promoter
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72627pDECKO_TFRC_C (with mCherry)Expresses two gRNAs targeting the TFRC promoter
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72628pDECKO_UCA1Expresses two gRNAs targeting the UCA1 promoter
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