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T. J. Kappock Lab: Additional fatty acid desaturase and acetylenase constructs
Kappock
Unpublished

Plasmids from Article

ID Plasmid Purpose  
73312pJK108Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)
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73313pJK137Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag
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73314pJK138Pas2p peroxisomal membrane targeting sequence
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73315pJK139Pas2p peroxisomal membrane biogenesis protein
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73316pJK141Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
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73317pJK142Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
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73336pJK125Encodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)
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73337pJK126Encodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)
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73363pJK275Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
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73366pJK276Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)
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73594pJK369Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope
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73595pJK370Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
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73596pJK371Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
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73597pJK372Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
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