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Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range.
Solscheid C, Kunzelmann S, Davis CT, Hunter JL, Nofer A, Webb MR
Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
78198pET22b_PstS_1Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
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78199pET22b_PstS_2Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
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78200pET22b_PstS_3Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
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