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Controlling mRNA stability and translation with the CRISPR endoribonuclease Csy4.
Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A
RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015 Sep 9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80591TR-GFPExpresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
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80592TR- ATG-HP-GFPExpresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
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80594TR-5'UTR-HP-GFPExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
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80596TR-GFP-3'UTR-HPExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 3'UTR. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
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80601TR-Csy4Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
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80602TR-Csy4-H29AExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
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80696TR-Csy4-R115A/R119AExpresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
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