Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Controlling mRNA stability and translation with the CRISPR endoribonuclease Csy4.
Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A
RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015 Sep 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
80591TR-GFPExpresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
80592TR- ATG-HP-GFPExpresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
80594TR-5'UTR-HP-GFPExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
80601TR-Csy4Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
80602TR-Csy4-H29AExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
80696TR-Csy4-R115A/R119AExpresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Antibodies from Article