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Comprehensive analysis of RNA-protein interactions by high-throughput sequencing-RNA affinity profiling.
Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT
Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 2014 May 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
53302EGFP-mOrange pHis-//Expresses His-tagged EGFP-mOrange fusion protein.
53303NELFE-mOrange pHis-//Expresses His-tagged NELF-E-mOrange fusion protein.
53304mOrange pHis-//Empty vector to express N-terminal His-mOrange-tagged fusion protein.
53305Tus pGST-//Expresses GST-tagged E.coli replication terminator protein Tus.

Antibodies from Article