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LOVTRAP: an optogenetic system for photoinduced protein dissociation.
Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM
Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
81009pTriEx-NTOM20-LOV2Expression of LOV2 anchored to mitochondria
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81010pTriEx-NTOM20-mVenus-Zdk1Expression of Zdk1 anchored to mitochondria
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81011pTriEx-NTOM20-mVenus-Zdk2Expression of Zdk2 anchored to mitochondria
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81012pTriEx-NTOM20-mVenus-Zdk3Expression of Zdk1 anchored to mitochondria
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81031pTriEx-mCherry-LOV2 C450AMammalian expression of the dark state of LOV2
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81033pTriEx-mCherry-LOVSDMammalian expression of the super-dark state of LOV2
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81035pTriEx-mCherry-LOV2 I427TMammalian expression of LOV I427T, a mutant with super short relaxation time
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81036pTriEx-mCherry-LOV2 V416Lmammalian expression of LOV V416L, a mutant with super long relaxation time
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81040pTriEx-mCherry-LOV2 V416Tmammalian expression of LOV V416T
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81041pTriEx-mCherry-LOV2mammalian expression for wt LOV2
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81042pTriEx-NLyn-Venus-Zdk1mammalian expression of plasma-membrane anchored Zdk1
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81044pTriEx-mCherry-LOV2 I427Vmammalian expression of LOV2 I427V
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81045pTriEx-mCherry-LOV2 V416Imammalian expression of LOV V416I
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81046pTriEx-mVenus-Zdk1-RhoA Q63Lmammalian expression of Zdk1 tagged RhoA Q63L
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81047pTriEx-mVenus-Zdk1mammalian expression of Zdk1
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81057pTriEx-mCherry-Zdk1mammalian expression of Zdk1
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81058pTriEx-mCherry-Zdk1-RhoA Q63Lmammalian expression of Zdk1 tagged RhoA Q63L
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81059pTriEx-mCherry-Zdk1-Rac1 Q61Lmammalian expression of Zdk1 tagged Rac1 Q61L
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81060pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain
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81129pTriEx-mCherry-Zdk1-TIAM1 DH/PHmammalian expression of Zdk1 tagged Tiam1 Dh/PH
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81133pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain
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81137pTriEx-mVenus-Zdk1-Rac1 Q61Lmammalian expression of Zdk1 tagged Rac1 Q61L
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