LOVTRAP: an optogenetic system for photoinduced protein dissociation.
Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM
Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. PubMed Article
Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM
Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 81009 | pTriEx-NTOM20-LOV2 | Expression of LOV2 anchored to mitochondria |
| 81010 | pTriEx-NTOM20-mVenus-Zdk1 | Expression of Zdk1 anchored to mitochondria |
| 81011 | pTriEx-NTOM20-mVenus-Zdk2 | Expression of Zdk2 anchored to mitochondria |
| 81012 | pTriEx-NTOM20-mVenus-Zdk3 | Expression of Zdk1 anchored to mitochondria |
| 81031 | pTriEx-mCherry-LOV2 C450A | Mammalian expression of the dark state of LOV2 |
| 81033 | pTriEx-mCherry-LOVSD | Mammalian expression of the super-dark state of LOV2 |
| 81035 | pTriEx-mCherry-LOV2 I427T | Mammalian expression of LOV I427T, a mutant with super short relaxation time |
| 81036 | pTriEx-mCherry-LOV2 V416L | mammalian expression of LOV V416L, a mutant with super long relaxation time |
| 81040 | pTriEx-mCherry-LOV2 V416T | mammalian expression of LOV V416T |
| 81041 | pTriEx-mCherry-LOV2 | Mammalian or insect expression for wt LOV2 |
| 81042 | pTriEx-NLyn-Venus-Zdk1 | mammalian expression of plasma-membrane anchored Zdk1 |
| 81044 | pTriEx-mCherry-LOV2 I427V | mammalian expression of LOV2 I427V |
| 81045 | pTriEx-mCherry-LOV2 V416I | mammalian expression of LOV V416I |
| 81046 | pTriEx-mVenus-Zdk1-RhoA Q63L | mammalian expression of Zdk1 tagged RhoA Q63L |
| 81047 | pTriEx-mVenus-Zdk1 | mammalian expression of Zdk1 |
| 81057 | pTriEx-mCherry-Zdk1 | mammalian expression of Zdk1 |
| 81058 | pTriEx-mCherry-Zdk1-RhoA Q63L | mammalian expression of Zdk1 tagged RhoA Q63L |
| 81059 | pTriEx-mCherry-Zdk1-Rac1 Q61L | mammalian expression of Zdk1 tagged Rac1 Q61L |
| 81060 | pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1 | mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain |
| 81129 | pTriEx-mCherry-Zdk1-TIAM1 DH/PH | mammalian expression of Zdk1 tagged Tiam1 Dh/PH |
| 81133 | pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1 | mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain |
| 81137 | pTriEx-mVenus-Zdk1-Rac1 Q61L | mammalian expression of Zdk1 tagged Rac1 Q61L |