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Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, Fodor E, Welker E
Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
PubMed

Plasmids from Article

ID Plasmid Purpose  
74041pTE4396Expresses human codon-optimized AsCpf1 and As crRNA.
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74042pTE4398Expresses human codon-optimized LbCpf1 and Lb crRNA.
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80441WN10151Expresses human codon-optimized dead (832A) LbCpf1
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89050pTE4254Expresses St chimeric gRNA and human codon-optimized StCas9
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89051pKS7107Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
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89052pWN10042GF-ori-FP plasmid for GFxFP assay
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