pKS7107
(Plasmid #89051)

• Purpose: Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
• Depositing Lab: Ervin Welker
• Publication: Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.

Backbone

• Vector backbone: pSimpleII
• Backbone size w/o insert (bp): 3756
• Total vector size (bp): 9305
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Nm crRNA
• Alt name: Neisseria meningitidis CRISPR RNA
• Insert Size (bp): 51
• Promoter: human U6

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: AAAACAGGAAGGCAAAATGC
• 3′ sequencing primer: gggagatctcccgatccgtc

Gene/Insert 2

• Gene/Insert name: Nm tracrRNA
• Alt name: Neisseria meningitidis tracrRNA
• Insert Size (bp): 105
• Promoter: human U6

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: AAAACAGGAAGGCAAAATGC
• 3′ sequencing primer: gggagatctcccgatccgtc

Gene/Insert 3

• Gene/Insert name: hNmCas9
• Alt name: human codon-optimized Neisseria meningitidis Cas9 nuclease
• Promoter: EF1a
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on insert)
  • HA tag (C terminal on insert)
  • NLS (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: gactgaagttaggccagc
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry