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High-performance probes for light and electron microscopy.
Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger LL
Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 2015 Apr 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
59756pCAG_smFP FLAGencodes multiple copies of an epitope tag FLAG
59757pCAG_smFP Mycencodes multiple copies of an epitope tag Myc
59758pCAG_smFP V5encodes multiple copies of an epitope tag V5
59759pCAG_smFP HAencodes multiple copies of an epitope tag HA
59760pCAG_mRuby2_smFP FLAGencodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)
59761pCAG_mRuby2_smFP OLLASencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)
98925pAAV.CAG.Ruby2sm-Flag.WPRE.SV40 AAV expression of Ruby2 spaghetti monster FLAG construct
98926pAAV.CAG.GFPsm-myc.WPRE.SV40 AAV expression of GFP spaghetti monster myc construct
98927pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Cre dependent AAV expression of GFP spaghetti monster myc construct
98928pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

Antibodies from Article