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A programmable Cas9-serine recombinase fusion protein that operates on DNA sequences in mammalian cells.
Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
81203pHu6-gRNA-NT1hU6 expression of gRNA NT1
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81204pCALNL-eGFP-NT1-6-gix-6-NT1contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid
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81205pGinB-8X(GGS)-dCas9-FLAG-NLSCMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus
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81206pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecRCMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance
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81207pHU6_gRNA_Ch10-rev2for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 5' of gix site)
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81208pHU6_gRNA_Ch10-rev1for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)
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81209pHU6_gRNA_Ch10-for2for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)
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81210pHU6_gRNA_Ch10-for1for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)
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81211pCALNL_Ch5_155183064-155183141pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)
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81212pCALNL_Ch12_62418577-62418652pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)
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81213pCALNL_chr13_102010574-102010650pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)
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81214pHU6_Ch5_155183064-gRNA-forpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporter
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81215pHU6_Ch5_155183064-gRNA-revpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter
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81216pHU6_Ch12_62418577-gRNA-forpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch12 reporter
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81217pHU6_Ch12_62418577-gRNA-revpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter
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81218pHU6_Ch13_102010574-gRNA-forpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter
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81219pHU6_Ch13_102010574-gRNA-revpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch13 reporter
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81220pHU6_chr12_FAM19A2-up-gRNA-revpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locus
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81221pHU6_chr12_FAM19A2-up-gRNA-forpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locus
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81222pHU6_chr12_FAM19A2-down-gRNA-revpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locus
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81223pHU6_chr12_FAM19A2-down-gRNA-forpHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locus
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81224pCALNL_Ch10pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10
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81226pCALNL-eGFP-Esp3ICloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites
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