A programmable Cas9-serine recombinase fusion protein that operates on DNA sequences in mammalian cells.
Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
Nucleic Acids Res. 2016 Aug 11. pii: gkw707. PubMed Article
Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
Nucleic Acids Res. 2016 Aug 11. pii: gkw707. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 81203 | pHu6-gRNA-NT1 | hU6 expression of gRNA NT1 |
| 81204 | pCALNL-eGFP-NT1-6-gix-6-NT1 | contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid |
| 81205 | pGinB-8X(GGS)-dCas9-FLAG-NLS | CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus |
| 81206 | pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR | CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance |
| 81207 | pHU6_gRNA_Ch10-rev2 | for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 5' of gix site) |
| 81208 | pHU6_gRNA_Ch10-rev1 | for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site) |
| 81209 | pHU6_gRNA_Ch10-for2 | for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site) |
| 81210 | pHU6_gRNA_Ch10-for1 | for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site) |
| 81211 | pCALNL_Ch5_155183064-155183141 | pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38) |
| 81212 | pCALNL_Ch12_62418577-62418652 | pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38) |
| 81213 | pCALNL_chr13_102010574-102010650 | pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38) |
| 81214 | pHU6_Ch5_155183064-gRNA-for | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporter |
| 81215 | pHU6_Ch5_155183064-gRNA-rev | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter |
| 81216 | pHU6_Ch12_62418577-gRNA-for | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch12 reporter |
| 81217 | pHU6_Ch12_62418577-gRNA-rev | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter |
| 81218 | pHU6_Ch13_102010574-gRNA-for | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter |
| 81219 | pHU6_Ch13_102010574-gRNA-rev | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch13 reporter |
| 81220 | pHU6_chr12_FAM19A2-up-gRNA-rev | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locus |
| 81221 | pHU6_chr12_FAM19A2-up-gRNA-for | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locus |
| 81222 | pHU6_chr12_FAM19A2-down-gRNA-rev | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locus |
| 81223 | pHU6_chr12_FAM19A2-down-gRNA-for | pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locus |
| 81224 | pCALNL_Ch10 | pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10 |
| 81226 | pCALNL-eGFP-Esp3I | Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites |