Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins.
Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046. PubMed Article
Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84354 | CyRFP1-C1 | Expresses CyRFP1 fluorescent protein in an empty backbone |
| 84355 | mCyRFP1-C1 | Expresses mCyRFP1 fluorescent protein in an empty backbone |
| 84356 | pCAG-CyRFP1 | Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter |
| 84357 | AAV-FLEX-CAG-CyRFP1 | Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent) |
| 84358 | mCyRFP1-RhoA | fusion protein of RhoA, FRET donor |
| 84359 | mMaroon-RTK-mMaroon | FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon |
| 84360 | mCyRFP1-Cdc42 | fusion protein of Cdc42, FRET donor |
| 84361 | mMaroon-PAK-mMaroon | FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon |
| 84486 | mCyRFP1-mMaroon | Direct FRET pair Fusion mCyRFP1-mMaroon |
| 84545 | pNCS-mCyRFP1 | Bacterial expression of mCyRFP1 |
| 84546 | pNCS-CyRFP1 | Bacterial expression of CyRFP1 |