Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells.
Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T
Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85130 | pCAG-AMPH1-BAR-mCherry | BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry |
| 85131 | pLV-Ftractin-mCherry | lentiviral expression of an F-actin live-cell marker (mCherry) |
| 85132 | pLV-EF1a-IRES-Puro | lentiviral backbone |
| 85133 | pLV-EF1a-IRES-Blast | lentiviral backbone |
| 85134 | pLV-EF1a-IRES-Hygro | lentiviral backbone |
| 85139 | pLV-EF1a-IRES-Neo | lentiviral backbone |
| 85140 | pLV-hTERT-IRES-hygro | Lentiviral expression of hTERT, used to create immortalized cell lines |
| 85143 | pLV-CDH5-mCitrine-IRES-puro | lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine |
| 85144 | pLV-CDH5-mRuby3-IRES-puro | lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3 |
| 85145 | pLV-mTurquoise-MLC-IRES-neo | lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise |
| 85146 | pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast | lentiviral expression of a stoichiometric F-actin and myosin light chain reporter |
| 85152 | pCAG-mCherry-FKBP-GEF(ARHGEF1) | acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB |
| 85156 | pCAG-mCherry-FKBP-GEF(TIAM1) | acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB |