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Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.
Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha S, Kotter M, Vallier L
Development. 2016 Dec 1;143(23):4405-4418.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86695pAAV-Puro_siKDTetracycline inducible expression of shRNA targeted to AAVS1 locus
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86696pAAV-Puro_siKOTetracycline inducible expression of guide RNA targeted to AAVS1 locus
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86697pAAV-Puro_siKO-2TOTetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus
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86698pAAV-Neo_CAG-Cas9Cas9 expression vector for targeting to the AAVS1 locus
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