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Engineering extrinsic disorder to control protein activity in living cells.
Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, Dokholyan NV, Hahn KM
Science. 2016 Dec 16;354(6318):1441-1444.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86974mCherry-PI(WT)-Vav2(DPZ)PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2
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86977mCherry-PA(WT)-Vav2PI(DM): Photo-activatable/light sensitive
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86978pTriex-mVenus-PI-RhoA(Q63L)PI(DM): Photo-activatable/light sensitive
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87356pBabe-PI(WT)-Src(WT)-mCherryPI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type
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87357pBabe-PI(WT)-Src(YF)-mCherryPI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation
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87358pBabe-PI(WT)-Src(KD)-mCherryPI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation
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87550pBabe-PI(WT)-Rac1(WT)-mCherryPI(WT): Photo-inhibitable/blue light sensitive Rac1
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87553pBabe-PI(WT)-Rac1(Q61L)-mCherryPI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1
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87589PI(DM)-Rac1(WT)dc1.FLAREPI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor
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87590PI(LM)-Rac1(WT)dc1.FLAREPI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor
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91873pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2Photo-activatable/light sensitive Vav2
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91874pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2
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91875mCherry-uniRapR-P-Vav2(DPZ)Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2
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91876pBabe-tetoff-mVenus-PICdc42PI(WT): Photo inhibitable (blue light sensitive)
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