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In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.
Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway EM, Zhang K, Belmonte JC
Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87108pCAG-1BPNLS-Cas9-1BPNLSBPNLS-Cas9-BPNLS expression plasmid
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87109pCAG-1BPNLS-Cas9-1BPNLS-2AGFPBPNLS-Cas9-BPNLS-2AGFP expression plasmid
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87110pCAGmCherry-gRNAgRNA cloning vector with CAGmCherry
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87111Tubb3gRNA-mCherrymouse Tubb3 target gRNA expression plasmid with CAGmCherry
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87112pTubb3-MCminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI
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87113pAi14-luc-MCminicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI
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87114pAi14-GFPNLS-MCminicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI
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87115pAAV-nEFCas9SpCas9 expression AAV backbone plasmid under control of nEF promoter
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87116pAAV-mTubb3AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI
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87117pAAV-Ai14-HITIAAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI
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87118pAAV-Ai14-lucAAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI
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87119pAAV-rMERTK-HITIGene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI
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