A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large.
Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den Heuvel S, Heck AJ, Boxem M
BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x. PubMed Article
Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den Heuvel S, Heck AJ, Boxem M
BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73084 | pMB37 | Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter |
| 73085 | pMB71 | Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter |
| 73086 | pMB73 | Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter |
| 73087 | pMB41 | N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering |
| 73088 | pMB72 | C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering |
| 75026 | pJJR50 | U6 promoter driven flipped + extended sgRNA expression vector |
| 79971 | rgef-1p::NmBirA | encodes E.coli biotin-ligase under neuronal promotor for C.elegans |