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An Argonaute phosphorylation cycle promotes microRNA-mediated silencing.
Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT
Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23.
PubMed Article

Plasmids from Article

ID Plasmid  
91975MSCV-EGFP
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91976MSCV-EGFP-Bulged-miR-19
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91977LcV2-Hygro
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91978pLJM1-FH-AGO2-WT
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91979pLJM1-FH-AGO2-5xA
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91980pLJM1-Empty
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91981pLJM1-FH-AGO2-5xE
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91982pLJM1-FH-AGO2-S828A
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91983pLJM1-FH-AGO2-D358K
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91984pLJM1-FH-AGO2-R438E
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91985pLJM1-FH-AGO2-K525E
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91986MSCV-Puro-FH-AGO2-WT
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91987MSCV-Puro-FH-AGO2-S824A
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91988MSCV-Puro-FH-AGO2-T830A
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91989MSCV-Puro-FH-AGO2-S831A
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91990MSCV-Puro-FH-AGO2-S834A
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91991MSCV-Puro-FH-AGO2-S828A
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91992MSCV-Puro-FH-AGO2-5xA
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91993MSCV-Puro-FH-AGO2-5xE
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91994MSCV-Puro-FH-AGO2-S824E
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91995MSCV-Puro-FH-AGO2-S828E
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91996MSCV-Puro-FH-AGO2-T830E
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91997MSCV-Puro-FH-AGO2-S831E
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91998MSCV-Puro-FH-AGO2-S834E
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91999MSCV-Puro-FH-AGO2-S824E-S828E
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92000MSCV-Puro-FH-AGO2-S824E-S831E
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92001MSCV-Puro-FH-AGO2-S828E-S831E
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92002MSCV-Puro-FH-AGO2-S824E-S828E-S831E
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92003MSCV-Puro-FH-AGO2-S824A-T830A
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92004pGL3-BoxB
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92005pcDNA3.1+_Lambda-N-HA-Peptide
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92006pcDNA3.1+_FH-AGO2-WT
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92007pcDNA3.1+_FH-AGO2-5xA
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92008pcDNA3.1+_FH-AGO2-5xE
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92009pcDNA3.1+_LN-HA-AGO2-WT
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92010pcDNA3.1+_LN-HA-AGO2-5xA
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92011pcDNA3.1+_LN-HA-AGO2-5xE
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92012pcDNA3.1+_V5-ANKRD52
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92013pCAGIG_V5-PPP6C
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92014pCAGImC_V5-CSNK1A1
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92015pCAGImC_Empty
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