Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
Zhao Y, Boeke JD
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363. PubMed Article
Zhao Y, Boeke JD
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98404 | pYZ033 | Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1 |
| 98405 | pYZ145 | Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion. |
| 98406 | pYZ146 | Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe |
| 98407 | pYZ149 | Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion. |
| 98408 | pYZ164 | Cas9-gRNA his3 plasmid for his3 deletion in S. pombe |
| 98409 | pYZ172 | Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion. |
| 98410 | pYZ173 | Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe |
| 98411 | pYZ155 | Pombe Expression Vector - nmt1 promoter with Sp.ura4 marker |
| 98412 | pYZ156 | Pombe Expression Vector - nmt41 promoter with Sp.ura4 marker |
| 98413 | pYZ157 | Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker |
| 98414 | pYZ158 | Pombe Expression Vector - nmt1 promoter with Sp.leu1 marker |
| 98415 | pYZ159 | Pombe Expression Vector - nmt41 promoter with Sp.leu1 marker |
| 98416 | pYZ160 | Pombe Expression Vector - nmt81 promoter with Sp.leu1 marker |
| 98418 | pYZ165 | Pombe Expression Vector - nmt1 promoter with Sp.his3 marker |
| 98419 | pYZ166 | Pombe Expression Vector - nmt41 promoter with Sp.his3 marker |
| 98420 | pYZ167 | Pombe Expression Vector - nmt81 promoter with Sp.his3 marker |
| 98421 | pYZ174 | Pombe Expression Vector - nmt1 promoter with Sp.lys9 marker |
| 98422 | pYZ175 | Pombe Expression Vector - nmt41 promoter with Sp.lys9 marker |
| 98423 | pYZ176 | Pombe Expression Vector - nmt81 promoter with Sp.his3 marker |
| 98424 | pYZ182 | Pombe Expression Vector - nmt1 promoter with empty BsaI-pad - no marker |
| 98425 | pYZ183 | Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker |
| 98426 | pYZ184 | Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker |
| 98427 | pYZ189 | Pombe General Purpose Vector - with empty BsaI-pad for new potential markers |
| 98428 | pYZ190 | Pombe General Purpose Vector - with Sp.ura4 marker |
| 98429 | pYZ191 | Pombe General Purpose Vector - with Sp.leu1 marker |
| 98430 | pYZ192 | Pombe General Purpose Vector - with Sp.his3 marker |
| 98431 | pYZ193 | Pombe General Purpose Vector - with Sp.lys9 marker |
| 102686 | pYZ292 | S. pombe gRNA entry vector with Cas9 - ScLEU2 marker |
| 102687 | pYZ293 | S. pombe gRNA entry vector with Cas9 - Sp leu1 marker |
| 102688 | pYZ294 | S. pombe gRNA entry vector with Cas9 - KanMX marker |
| 102689 | pYZ300 | S. pombe gRNA entry vector with Cas9 - Sp his3 marker |
| 102690 | pYZ301 | S. pombe gRNA entry vector with Cas9 - Sp lys9 marker |