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A multi-purpose toolkit to enable advanced genome engineering in plants.
Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
90997pMOD_A0000Module A, Promoter: none, Gene: none, Terminator: none
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90998pMOD_A0101Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP
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91000pMOD_A0103Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP
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91001pMOD_A0108Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP
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91002pMOD_A0201Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP
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91003pMOD_A0202Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP
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91004pMOD_A0208Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP
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91005pMOD_A0301Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP
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91006pMOD_A0302Module A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSP
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91007pMOD_A0308Module A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSP
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91009pMOD_A0402Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP
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91010pMOD_A0408Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP
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91011pMOD_A0501Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9, Terminator: HSP
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91012pMOD_A0502Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP
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91013pMOD_A0503Module A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSP
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91014pMOD_A0508Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP
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91016pMOD_A0601Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP
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91018pMOD_A0608Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP
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91019pMOD_A0701Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP
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91021pMOD_A0708Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP
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91022pMOD_A0801Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP
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91023pMOD_A0802Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP
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91024pMOD_A0808Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP
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91025pMOD_A0901Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP
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91026pMOD_A0902Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSP
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91027pMOD_A0908Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP
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91029pMOD_A1002Module A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)
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91030pMOD_A1010Module A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)
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91031pMOD_A1110Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP
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91032pMOD_A1111Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP
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91033pMOD_A1210Module A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSP
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91034pMOD_A1310Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP
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91035pMOD_A1410Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP
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91036pMOD_A1510Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP
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91037pMOD_A1511Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP
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91038pMOD_A1610Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP
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91039pMOD_A1710Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP
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91040pMOD_A1810Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP
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91041pMOD_A1910Module A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSP
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91042pMOD_A3001Module A, Promoter: 35S, Gene: GFP, Terminator: HSP
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91043pMOD_A3003Module A, Promoter: CmYLCV, Gene: GFP, Terminator: HSP
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91044pMOD_A3010Module A, Promoter: ZmUbi, Gene: GFP, Terminator: HSP
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91045pMOD_A3012Module A, Promoter: PvUbi1, Gene: GFP, Terminator: HSP
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91046pMOD_A3013Module A, Promoter: PvUbi2, Gene: GFP, Terminator: HSP
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91047pMOD_A3014Module A, Promoter: Nos, Gene: GFP, Terminator: HSP
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91048pMOD_A3101Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)
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91049pMOD_A3301Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)
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91050pMOD_A3501Module A, Promoter: 35S, Gene: dAtCas9-VPR, Terminator: HSP
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91051pMOD_A3601Module A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSP
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91052pMOD_A3701Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSP
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91053pMOD_A3801Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VP64, Terminator: HSP
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91054pMOD_A3910Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP
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91055pMOD_A4010Module A, Promoter: ZmUbi, Gene: dTaCas9-VP64, Terminator: HSP
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91056pMOD_A4110Module A, Promoter: ZmUbi, Gene: Csy4-P2A-dTaCas9-VPR, Terminator: HSP
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91057pMOD_A4210Module A, Promoter: Zmubi, Gene: Csy4-P2A-dTaCas9-VP64, Terminator: HSP
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91058pMOD_B0000Module B, Promoter: none, Gene: none, Terminator: none
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91059pMOD_B2000Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
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91060pMOD_B2101Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
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91061pMOD_B2103Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
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91062pMOD_B2103bModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
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91064pMOD_B2112Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
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91065pMOD_B2121Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
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91066pMOD_B2203Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C
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91067pMOD_B2301Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
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91068pMOD_B2303Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
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91069pMOD_B2309Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
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91070pMOD_B2312Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
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91071pMOD_B2403Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S
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91072pMOD_B2515Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
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91073pMOD_B2515bModule B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III
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91074pMOD_B2517Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
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91075pMOD_B2518Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
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91076pMOD_B2519Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
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91077pMOD_B2520Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
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91078pMOD_B2615Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
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91079pMOD_B3200Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
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91080pMOD_B3400Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
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91081pMOD_C0000Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none
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91082pMOD_C2200Module C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35S
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91083pMOD_C2515Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
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91084pMOD_C2516Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
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91085pMOD_C2516bModule C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III
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91086pMOD_C2517Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
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91087pMOD_C2518Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
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91088pMOD_C2519Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
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91089pMOD_C2520Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
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91090pMOD_C2616Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
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91091pMOD_C2806Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91092pMOD_C2813Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91093pMOD_C2906Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91094pMOD_C3001Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91095pMOD_C3003Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91096pMOD_C3006Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91098pMOD_C3010Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91099pMOD_C3012Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91100pMOD_C3013Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
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91101pMOD_C3106Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
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91102pMOD_C3306Module C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
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91103pMOD_C4300Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: none
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91105pTRANS_201Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV
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91106pTRANS_202Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: ToLCV
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91108pTRANS_210Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none
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91109pTRANS_210dTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none
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91110pTRANS_211Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV
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91111pTRANS_212Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV
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91112pTRANS_213Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV
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91113pTRANS_220Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none
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91114pTRANS_220dTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none
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91115pTRANS_221Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV
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91116pTRANS_222Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV
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91117pTRANS_223Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDV
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91118pTRANS_230Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: none
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91120pTRANS_231Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV
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91121pTRANS_232Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV
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91122pTRANS_233Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV
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91125pTRANS_253Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV
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91126pTRANS_260dTransformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none
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91127pTRANS_263Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV
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91128pDIRECT_21ADirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II
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91129pDIRECT_21BDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II
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91130pDIRECT_21CDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
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91131pDIRECT_21DDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II
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91132pDIRECT_21JDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:hpt II
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91133pDIRECT_22ADirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II
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91135pDIRECT_22CDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
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91136pDIRECT_22DDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
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91137pDIRECT_22JDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:npt II
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91138pDIRECT_23ADirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar
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91140pDIRECT_23CDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar
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91141pDIRECT_23DDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar
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91142pDIRECT_23JDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:bar
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91143pDIRECT_25FDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II
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91144pDIRECT_25GDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II
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91145pDIRECT_25HDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
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91146pDIRECT_25IDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
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91147pDIRECT_25KDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:hpt II
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91149pDIRECT_26GDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
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91150pDIRECT_26HDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
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91152pDIRECT_26KDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:bar
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91153pDIRECT_37JDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II
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91154pDIRECT_38JDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt II
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91155pDIRECT_39JDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:bar
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91156pAtCas9_1Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II
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91158pAtCas9_3Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar
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91159pAtCas9-D10A_1Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II
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91160pAtCas9-D10A_2Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II
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91161pAtCas9-D10A_3Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar
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91162pAtCas9-H840A_1Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II
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91163pAtCas9-H840A_2Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II
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91165pAtCas9-dead_1Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II
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91166pAtCas9-dead_2Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II
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91167pAtCas9-dead_3Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar
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91168pTaCas9_5Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II
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91169pTaCas9_6Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar
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91170pTaCas9-D10A_5Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II
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91171pTaCas9-D10A_6Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar
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91172pTaCas9-H840A_5Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II
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91173pTaCas9-H840A_6Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar
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91174pTaCas9-dead_5Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II
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91175pTaCas9-dead_6Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:bar
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91176pTC102a derivative of pDIRECT_37J with translation initiation sequence optimized for dicots
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91178pTC130BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)
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91179pSC1T-DNA vector expressing TALENs targeting Medtr4g020620 and Medtr2g013650A
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91180pSC2 T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)
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91181pSC3T-DNA vector for targeted deletion of 58kb region in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)
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91182pSC4T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)
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91183pJG363BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)
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91185pJG367BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)
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91187pJG370BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)
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91188pJG380BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)
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91189pJG382BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)
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91194pJG484WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)
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91195pJG486WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)
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91196pJG488WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)
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91197pJG493WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)
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91198pTRANS_100Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none
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91199pTRANS_101Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV
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91200pTRANS_102Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: ToLCV
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91202pTRANS_110Transformation, Type: non-T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none
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91203pTRANS_120Transformation, Type: non-T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none
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91204pTRANS_130Transformation, Type: non-T-DNA, Plant Selection: 2x35S:bar, Viral Replication: none
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91205pTRANS_150Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none
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91206pTRANS_160Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none
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91207pDIRECT_10ADirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none
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91209pDIRECT_10EDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none
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91210pTC303protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)
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91211pTC363protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters)
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91212pAH750protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)
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91213pTC364protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)
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91214pTC365protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)
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91215pTC366protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)
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91216pTC362 protoplast vector for targeted deletion of 6 genes in tomato
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91218pTC441protoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)
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91219pTC437protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)
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91220pTC391protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)
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91221pTC393protoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)
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91222pTC436protoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)
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91223pTC392protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)
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91224pTC394 protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)
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91225pTC440protoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)
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