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Real-time observation of light-controlled transcription in living cells.
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
103797pCIBN-tagBFP-TetRBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
103798pCIBN-EYFP-TetRBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
103799pCIBN-tagBFP-hLaminB1BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light
103800pCIBN-EYFP-hLaminB1BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light
103801pCIBN-tagBFP-hTRF1BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
103802pCIBN-EYFP-hTRF1BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
103803pCIBN-tagBFP-hTRF2BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
103804pCIBN-EYFP-hTRF2BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
103805pCIBN-tagBFP-hNCLBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
103806pCIBN-EYFP-hNCLBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
103807pCIBN-tagBFP-hPMLIIIBLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light
103808pCIBN-EYFP-hPMLIIIBLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light
103809pCIBN-TetR-tagRFP-T'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
103810pCIBN-LacI-tagRFP-T'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
103811pCIBN-hTRF1-tagRFP-TBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
103812pCIBN-hTRF2-tagRFP-TBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
103813pCIBN-TetRBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
103814pCIBN-LacIBLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
103815pCIBN-EYFPSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue light
103816pCIBN-mCherrySoluble BLInCR construct that can interact with PHR constructs upon illumination with blue light
103817pPHR-EYFPSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
103818pPHR-iRFP713Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
103819pPHR-mCherry-NLSSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
103820pPHR-EYFP-NLSSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
103821pPHR-mCherry-NLS-VP16Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
103822pPHR-EYFP-NLS-VP16Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
103823pPHR-iRFP713-NLS-VP16Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
103824pPHR-mCherry-hPMLIIISoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
103825pPHR-EYFP-hPMLIIISoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
103826pPHR-EYFP-hGCN5Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
103827pPHR-EYFP-hGCN5-NLSSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
103828pPHR-EYFP-hGCN5mutSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
103829pPHR-EYFP-hHP1βSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
103830pPHR-mCherry-hHP1βSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
103831ptagRFP-MS2coatProteinLive cell reader for MS2 loop RNA production
103832pmCherry-MS2coatProteinLive cell reader for MS2 loop RNA production
103833pmKate2-MS2coatProteinLive cell reader for MS2 loop RNA production
103834pEGFP-TetR-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays
103835ptagBFP-TetR-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays
103836pEGFP-LacI-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays
103837ptagBFP-LacI-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays
103838pNLS-TetR-GFPFlourescent marker for tetO arrays
103839ptagBFP-LacIFlourescent marker for lacO arrays
103840pCFP-LacI-NLSFlourescent marker for lacO arrays

Antibodies from Article