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Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling.
Uda Y, Goto Y, Oda S, Kohchi T, Matsuda M, Aoki K
Proc Natl Acad Sci U S A. 2017 Oct 24. pii: 201707190. doi: 10.1073/pnas.1707190114.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
100280pCAGGS-PHFFExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
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100281pCAGGS-hPhyB-mCherry-HRasCTExpresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
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100282pCAGGS-hPhyB621-mCherry-HRasCTExpresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells
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100283pCAGGS-PIF3-mEGFPExpresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells
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100285pCAGGS-PHFF-sh-hBVRAExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
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100286pCAGGS-PHFF-sh-mBVRAExpression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells
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100540pCAGGS-hPhyBY276H-mCherry-HRasCTExpresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
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