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A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis PL, Jeffree RL, Johns TG, Boyd AW
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
67501pF CAG luc puroConstitutive expression of firefly luciferase in mammalian cells
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67502pF CAG luc hygroConstitutive expression of firefly luciferase in mammalian cells
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67503pF CAG luc-EGFP-cre puroConstitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells
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98290lentiCRISPRv2 puroVariant of lentiCRISPRv2 that confers puromycin resistance
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98291lentiCRISPRv2 hygroVariant of lentiCRISPRv2 that confers hygromycin resistance
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98292lentiCRISPRv2 neoVariant of lentiCRISPRv2 that confers G418 resistance
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98293lentiCRISPRv2 blastVariant of lentiCRISPRv2 that confers blasticidin S resistance
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98294pF CAG luc IRES neoFor constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene
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104990lenti-sgRNA puroThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
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104991lenti-sgRNA hygroThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
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104992lenti-sgRNA neoThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
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104993lenti-sgRNA blastThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
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104994lenti-SpCas9 puroThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.
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104995lenti-SpCas9 hygroThis lentiviral construct delivers hSpCas9 and hygromycin resistance.
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104996lenti-SpCas9 neoThis lentiviral construct delivers hSpCas9 and G418 resistance.
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104997lenti-SpCas9 blastThis lentiviral construct delivers hSpCas9 and blasticidin S resistance.
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108175pF CAG BFP2 IRES neoExpresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418
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