RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors.
Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA
Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4. PubMed Article
Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA
Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106219 | AAV CMV-dSaCas9-KRAB-bGHpA | AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor |
| 106248 | AAV-Stuffer | Destination vector for U6::gRNA expression cassette |
| 106249 | pLV hUbC-dSaCas9-KRAB-T2A-PuroR | Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB |