AAV-Stuffer
(Plasmid
#106248)
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Purpose(Empty Backbone) Destination vector for U6::gRNA expression cassette
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTR
- Backbone size (bp) 7000
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Modifications to backboneAdded stuffer sequence
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsSURE2 is also an acceptable E. coli growth strain
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Vector used as a landing pad to clone in U6-S. aureus guide RNA expression cassette using the KpnI and XbaI sites
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-Stuffer was a gift from Charles Gersbach (Addgene plasmid # 106248 ; http://n2t.net/addgene:106248 ; RRID:Addgene_106248) -
For your References section:
RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors. Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA. Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4. 10.1038/s41467-018-04048-4 PubMed 29700298
