Purpose(Empty Backbone) Destination vector for U6::gRNA expression cassette
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||106248||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7000
Modifications to backboneAdded stuffer sequence
Growth in Bacteria
- Cloning method Unknown
Vector used as a landing pad to clone in U6-S. aureus guide RNA expression cassette using the KpnI and XbaI sites
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-Stuffer was a gift from Charles Gersbach (Addgene plasmid # 106248 ; http://n2t.net/addgene:106248 ; RRID:Addgene_106248)
For your References section:RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors. Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA. Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4. 10.1038/s41467-018-04048-4 PubMed 29700298