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A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
St Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS
Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.
PubMed Article

Plasmids from Article

ID Plasmid  
109425A3Ai-Cas9n-UGI-NLS
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109426A3Bi-ctd-Cas9n-UGI-NLS
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109427plenti-CMV-mCherry-T2A-GFP
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109428ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)
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109429A3Bi-ctd E255A -Cas9n-UGI-NLS
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109430A3Ai E72A-Cas9n-UGI-NLS
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109431mCherry Codon 59 sgRNA
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109432Non-specific sgRNA
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