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A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs.
Huang C, Liang D, Tatomer DC, Wilusz JE
Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 10.1101/gad.314856.118.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110113Hy_pMT dati Exons 1-3Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA
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110114Hy_pMT dati MCS circfirefly_500Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells
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110115Hy_pMT dati MCS circfirefly_700Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells
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110116Hy_pMT dati MCS circfirefly_900Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells
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110117Hy_pMT dati MCS circfirefly_1100Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells
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110118Hy_pMT dati MCS circfirefly_1677Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells
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110119Hy_pMT dati MCS ExonExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing
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110120Hy_pMT Flag-Hel25E D193EExpresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity
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110121Hy_pMT Flag-Hel25E K91NExpresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity
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110122Hy_pMT Flag-Hel25E MUTExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity
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110123Hy_pMT Flag-Hel25E WTExpresses Flag-tagged D. melanogaster Hel25E
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110124Hy_pMT Flag-UAP56 MUTExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity
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110125Hy_pMT Flag-UAP56 WTExpresses Flag-tagged human UAP56 (DDX39B)
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110126Hy_pMT Flag-URH49 MUTExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity
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110127Hy_pMT Flag-URH49 WTExpresses Flag-tagged human URH49 (DDX39A)
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Recombinant Antibodies from Article